Labbot and TdB Labs: A Fluorescent Fusion of Expertise

Discover the powerful synergy between Labbot and TdB Labs as we delve into the promising capabilities of the FARD20 pH probe. Take a look at the beautiful titration curves and read firsthand accounts from our specialists in a collaboration that unlocks novel pH readings in bioscience.

Published on
March 8, 2023
An abstract visualisation of the inner filter effect.
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Forging a Vibrant Collaboration: Labbot Meets TdB Labs' FARD20 Probe

A company that sells fluorescent dyes, and a company that sells an instrument to measure fluorescence can learn a great deal from each other. Especially if the dye is an innovative pH reporter and the instrument has a special knack for pH titrations. This insight sparked the collaboration between us and TdB labs, which started by us trying out their new FARD20 pH probe.

It turned out to be a great match, and with very little preparation we could run a titration to demonstrate the unique fluorescence fingerprint of FRAD20 at over 400 titration points ranging from pH 2.5 to 8.4.

Selected spectra of the FARD20 fluorescent pH indicator, excited at 488 nm. Recorded by Labbot in an automatic titration ranging from pH 2.5 to pH 8.4.
The ratio between the fluorescence peak at 600 nm and 520 nm as a function of pH (blue dots) and a fit (green dashes) to the Henderson–Hasselbalch equation.

Here is what our application specialist, Mattias, had to say about it:

"I had the opportunity to try out a newly launched pH probe from TdB Labs called FARD20. FARD20 is composed of a dextran molecule with two attached fluorophores: Antonia RedTM, which remains static and FITC, which changes as a function of pH. This gives the FARD molecule a unique fluorescent fingerprint, mapping directly to pH. FARD20 seemed like a great match for many Labbot applications since it unlocks pH readouts in samples where pH electrodes cannot be used, such as the interior of cells, samples that are incompatible with electrode surfaces, or samples with very small volumes.

Conversely, the high precision titration system and full spectrum fluorescent readouts of Labbot made FARD20 very easy to work with, and we could get some very nice titration data almost out of the box. We are always excited when we find systems that complement the Labbot instrument and extend the range of experiments our users can perform. We look forward to our continued exchange of knowledge with TdB Labs to help them achieve the best possible science".

TdB Labs Senior Research Scientist, Raffaello Papadakis, has also expressed his view of the collaboration:

"Our recent collaboration with Labbot demonstrated their exceptional quality of work, accuracy, and professionalism. They helped us understand and explain the dependency of one of our recently launched pH probe; FARD20 (a dual fluorescent labelled dextran derivative) on pH. Their innovative product and high-quality services have been exceptional. We are delighted to continue our collaboration with them and would highly recommend Labbot to all our peers in bioscience".

Raffaello Papadakis, Senior Research Scientist at TdB Labs

Conclusion

In the realm of pH readings, FARD20 expands the possibilities for Labbot users. It offers precise pH readouts without the necessity of electrode intrusion into the sample—a genuine game-changer!

Read more about the FARD20 pH probe on TdB Labs website.

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