Featuring Labbot

... and the prior generations of the instrument, called Probe Drum.
Journal of the American Chemical Society
May 17, 2021

Charge Regulation during Amyloid Formation of α-Synuclein

Tinna Pálmadóttir, Anders Malmendal, Thom Leiding, Mikael Lund & Sara Linse

Electrostatic interactions play crucial roles in protein function. Measuring pKa value perturbations upon complex formation or self-assembly of e.g. amyloid fibrils gives valuable information about the effect of electrostatic interactions in those processes.

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The Proceedings of the National Academy of Sciences (PNAS)
May 17, 2022

An aggregation inhibitor specific to oligomeric intermediates of Aβ42 derived from phage display libraries of stable, small proteins

Sara Linse, Pietro Sormanni, David J. O’Connell

The self-assembly of amyloid β peptide (Aβ) to fibrillar and oligomeric aggregates is linked to Alzheimer’s disease. Aβ binders may serve as inhibitors of aggregation to prevent the generation of neurotoxic species and for the detection of Aβ species.

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Analytical Biochemistry
May 15, 2009

Precise detection of pH inside large unilamellar vesicles using membrane-impermeable dendritic porphyrin-based nanoprobes

Thom Leiding, Kamil Górecki, Tomas Kjellman, Sergei A. Vinogradov, Cecilia Hägerhäll, Sindra Peterson Årsköld

Accurate real-time measurements of proton concentration gradients are pivotal to mechanistic studies of proton translocation by membrane-bound enzymes. Here we report a detailed characterization of the pH-sensitive fluorescent nanoprobe Glu3, which is well suited for pH measurements in microcompartmentalized biological systems.

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